Review



endo iv  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    New England Biolabs endo iv
    Endo Iv, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 302 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/endonuclease+iv/Endonuclease+IV/us12571016-1467-22-25
    Average 96 stars, based on 302 article reviews
    endo iv - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    Purification:

    Article Title: DMN-seq enriches DNA hypomethylated regions for biomarker discovery using 5-methylcytosine glycosylase
    Article Snippet: .. Subsequently, in 1 × DME reaction buffer (80 mM Tris–HCl, pH 8, 200 mM NaCl, 200 mM EDTA, 1 mM dithiothreitol (DTT), 400 μg/mL BSA) and a reaction volume of 30 μl, 50 ng samples were treated with 400 ng of DME, and the treatment reaction was carried out at 37 °C for 2 h. After purification with a DNA Clean & Concentrate kit (Zymo Research), each replicate was treated with endonuclease IV (NEB) and exonuclease III (NEB), sequentially, following the manufacturer’s protocols and heat denatured at 95 °C for 5 min to remove the fragments generated from DME excision that did not contain 5’-P. 5mC-containing fragments with 5’-P in each replicate were then ligated to a customized DME ssDNA adaptor (IDT, Additional file : Table S1) via the terminal deoxyribonucleotidyl transferase-assisted adenylate connector-mediated ssDNA (TACS) ligation method following the procedure described by Miura et al. [ ]. ..

    Article Title: DMN-seq enriches DNA hypomethylated regions for biomarker discovery using 5-methylcytosine glycosylase.
    Article Snippet: .. Subsequently, in 1x DME reaction buffer (80 mM Tris-HCl, pH 8, 200 mM NaCl, 200 mM EDTA, 1 mM dithiothreitol (DTT), 400 μg/mL BSA) and a reaction volume of 30 μl, 50 ng samples were treated with 400 ng of DME, and the treatment reaction was carried out at 37 °C for 2 h. After purification with a DNA Clean & Concentrate kit (Zymo Research), each replicate was treated with endonuclease IV (NEB) and exonuclease III (NEB), sequentially, following the manufacturer’s protocols and heat denatured at 95 °C for 5 min to remove the fragments generated from DME excision that did not contain 5’-P. AR TIC LE IN PR ES S 5mC-containing fragments with 5’-P in each replicate were then ligated to a customized DME ssDNA adaptor (IDT, Additional file 1: Table S1) via the terminal deoxyribonucleotidyl transferaseassisted adenylate connector-mediated ssDNA (TACS) ligation method following the procedure described by Miura et al [69]. ..

    Generated:

    Article Title: DMN-seq enriches DNA hypomethylated regions for biomarker discovery using 5-methylcytosine glycosylase
    Article Snippet: .. Subsequently, in 1 × DME reaction buffer (80 mM Tris–HCl, pH 8, 200 mM NaCl, 200 mM EDTA, 1 mM dithiothreitol (DTT), 400 μg/mL BSA) and a reaction volume of 30 μl, 50 ng samples were treated with 400 ng of DME, and the treatment reaction was carried out at 37 °C for 2 h. After purification with a DNA Clean & Concentrate kit (Zymo Research), each replicate was treated with endonuclease IV (NEB) and exonuclease III (NEB), sequentially, following the manufacturer’s protocols and heat denatured at 95 °C for 5 min to remove the fragments generated from DME excision that did not contain 5’-P. 5mC-containing fragments with 5’-P in each replicate were then ligated to a customized DME ssDNA adaptor (IDT, Additional file : Table S1) via the terminal deoxyribonucleotidyl transferase-assisted adenylate connector-mediated ssDNA (TACS) ligation method following the procedure described by Miura et al. [ ]. ..

    Article Title: DMN-seq enriches DNA hypomethylated regions for biomarker discovery using 5-methylcytosine glycosylase.
    Article Snippet: .. Subsequently, in 1x DME reaction buffer (80 mM Tris-HCl, pH 8, 200 mM NaCl, 200 mM EDTA, 1 mM dithiothreitol (DTT), 400 μg/mL BSA) and a reaction volume of 30 μl, 50 ng samples were treated with 400 ng of DME, and the treatment reaction was carried out at 37 °C for 2 h. After purification with a DNA Clean & Concentrate kit (Zymo Research), each replicate was treated with endonuclease IV (NEB) and exonuclease III (NEB), sequentially, following the manufacturer’s protocols and heat denatured at 95 °C for 5 min to remove the fragments generated from DME excision that did not contain 5’-P. AR TIC LE IN PR ES S 5mC-containing fragments with 5’-P in each replicate were then ligated to a customized DME ssDNA adaptor (IDT, Additional file 1: Table S1) via the terminal deoxyribonucleotidyl transferaseassisted adenylate connector-mediated ssDNA (TACS) ligation method following the procedure described by Miura et al [69]. ..

    Ligation:

    Article Title: DMN-seq enriches DNA hypomethylated regions for biomarker discovery using 5-methylcytosine glycosylase
    Article Snippet: .. Subsequently, in 1 × DME reaction buffer (80 mM Tris–HCl, pH 8, 200 mM NaCl, 200 mM EDTA, 1 mM dithiothreitol (DTT), 400 μg/mL BSA) and a reaction volume of 30 μl, 50 ng samples were treated with 400 ng of DME, and the treatment reaction was carried out at 37 °C for 2 h. After purification with a DNA Clean & Concentrate kit (Zymo Research), each replicate was treated with endonuclease IV (NEB) and exonuclease III (NEB), sequentially, following the manufacturer’s protocols and heat denatured at 95 °C for 5 min to remove the fragments generated from DME excision that did not contain 5’-P. 5mC-containing fragments with 5’-P in each replicate were then ligated to a customized DME ssDNA adaptor (IDT, Additional file : Table S1) via the terminal deoxyribonucleotidyl transferase-assisted adenylate connector-mediated ssDNA (TACS) ligation method following the procedure described by Miura et al. [ ]. ..

    Article Title: DMN-seq enriches DNA hypomethylated regions for biomarker discovery using 5-methylcytosine glycosylase.
    Article Snippet: .. Subsequently, in 1x DME reaction buffer (80 mM Tris-HCl, pH 8, 200 mM NaCl, 200 mM EDTA, 1 mM dithiothreitol (DTT), 400 μg/mL BSA) and a reaction volume of 30 μl, 50 ng samples were treated with 400 ng of DME, and the treatment reaction was carried out at 37 °C for 2 h. After purification with a DNA Clean & Concentrate kit (Zymo Research), each replicate was treated with endonuclease IV (NEB) and exonuclease III (NEB), sequentially, following the manufacturer’s protocols and heat denatured at 95 °C for 5 min to remove the fragments generated from DME excision that did not contain 5’-P. AR TIC LE IN PR ES S 5mC-containing fragments with 5’-P in each replicate were then ligated to a customized DME ssDNA adaptor (IDT, Additional file 1: Table S1) via the terminal deoxyribonucleotidyl transferaseassisted adenylate connector-mediated ssDNA (TACS) ligation method following the procedure described by Miura et al [69]. ..



    Similar Products

    96
    New England Biolabs endo iv
    Endo Iv, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/endonuclease+iv/Endonuclease+IV/us12571016-1467-22-25
    Average 96 stars, based on 1 article reviews
    endo iv - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    New England Biolabs endoiv
    Endoiv, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/endonuclease+iv/Endonuclease+IV/bio_rxiv__64898__2026__02__27__708379-82-52-53
    Average 96 stars, based on 1 article reviews
    endoiv - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    New England Biolabs neb endo iv
    Neb Endo Iv, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/endonuclease+iv/Endonuclease+IV/bio_rxiv__64898__2026__02__25__707755-369-50-50
    Average 96 stars, based on 1 article reviews
    neb endo iv - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    New England Biolabs endonuclease iv
    Endonuclease Iv, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/endonuclease+iv/Endonuclease+IV/pmc13097799-183-13-15
    Average 96 stars, based on 1 article reviews
    endonuclease iv - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    95
    New England Biolabs iv m0304
    Iv M0304, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/endonuclease+iv/Endonuclease+VIII/pmc12682131-357-23-19
    Average 95 stars, based on 1 article reviews
    iv m0304 - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    Image Search Results